Krypton in PDB 1c61: T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton

All present enzymatic activity of T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton:
3.2.1.17;

The structure of T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton, PDB code: 1c61 was solved by M.L.Quillin, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	60.00 / 2.00
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.238, 61.238, 97.050, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The structure of T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Krypton atom in the T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton (pdb code 1c61). This binding sites where shown within 5.0 Angstroms radius around Krypton atom.
In total 3 binding sites of Krypton where determined in the T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton, PDB code: 1c61:
Jump to Krypton binding site number: 1; 2; 3;

Krypton binding site 1 out of 3 in the T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton


Mono view


Stereo pair view

A full contact list of Krypton with other atoms in the Kr binding site number 1 of T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton within 5.0Å range: probe atom residue distance (Å) B Occ A:Kr500 b:13.8 occ:0.18 CD2 A:LEU118 3.5 17.0 1.0 CD2 A:LEU133 3.9 17.5 1.0 SD A:MET102 4.0 20.2 1.0 CB A:PHE114 4.0 18.2 1.0 CE A:MET102 4.0 19.4 1.0 CG1 A:VAL111 4.0 16.4 1.0 CD1 A:LEU121 4.0 19.2 1.0 CB A:SER117 4.2 12.7 1.0 CD1 A:LEU99 4.2 21.1 1.0 CG A:LEU118 4.2 20.8 1.0 CD2 A:PHE114 4.3 38.2 1.0 CG A:PHE114 4.4 22.1 1.0 N A:LEU118 4.6 14.2 1.0 KR A:KR501 4.6 20.0 0.1 O A:VAL111 4.7 17.5 1.0 CA A:LEU118 4.8 15.9 1.0 O A:PHE114 4.8 17.7 1.0 C A:SER117 4.8 14.8 1.0

Krypton binding site 2 out of 3 in the T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton


Mono view


Stereo pair view

A full contact list of Krypton with other atoms in the Kr binding site number 2 of T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton within 5.0Å range: probe atom residue distance (Å) B Occ A:Kr501 b:20.0 occ:0.09 CD1 A:LEU121 3.6 19.2 1.0 CB A:ALA98 3.7 11.5 1.0 CB A:ALA153 3.7 12.3 1.0 N A:LEU99 3.8 10.6 1.0 SD A:MET102 3.8 20.2 1.0 CA A:LEU99 4.0 11.5 1.0 CD1 A:LEU99 4.0 21.1 1.0 C A:ALA98 4.0 16.2 1.0 O A:ALA98 4.3 11.7 1.0 CB A:LEU99 4.3 16.9 1.0 CA A:ALA98 4.5 13.9 1.0 O A:ARG95 4.6 17.0 1.0 KR A:KR500 4.6 13.8 0.2 CD2 A:LEU121 4.7 13.2 1.0 CG A:LEU99 4.8 24.9 1.0 CG A:LEU121 4.8 16.1 1.0 CA A:ALA153 4.9 11.7 1.0 CG A:MET102 4.9 15.2 1.0 CD1 A:LEU133 5.0 14.0 1.0

Krypton binding site 3 out of 3 in the T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton


Mono view


Stereo pair view

A full contact list of Krypton with other atoms in the Kr binding site number 3 of T4 Lysozyme Mutant C54T/C97A/F153A in the Presence of 8 Atm Krypton within 5.0Å range: probe atom residue distance (Å) B Occ A:Kr502 b:20.0 occ:0.11 N A:ARG154 3.3 17.4 1.0 CB A:ALA153 3.4 12.3 1.0 C A:ALA153 3.5 9.3 1.0 CA A:ARG154 3.5 16.6 1.0 O A:TRP126 3.6 15.4 1.0 N A:ALA130 3.6 12.5 1.0 CB A:ALA129 3.6 9.9 1.0 O A:ALA153 3.7 15.1 1.0 CD1 A:LEU133 3.7 14.0 1.0 CA A:ALA130 3.8 13.9 1.0 CB A:ALA130 3.8 14.1 1.0 CD2 A:LEU121 3.9 13.2 1.0 CB A:ARG154 3.9 12.2 1.0 O A:ILE150 3.9 12.8 1.0 C A:ALA129 4.0 16.1 1.0 CA A:ALA153 4.1 11.7 1.0 CA A:ALA129 4.4 10.9 1.0 C A:TRP126 4.5 18.9 1.0 O A:ALA129 4.6 14.7 1.0 CG2 A:ILE150 4.7 17.7 1.0 CA A:TRP126 4.7 11.7 1.0 CG A:ARG154 4.7 15.9 1.0 C A:ILE150 4.9 19.4 1.0 C A:ARG154 5.0 17.7 1.0 CG A:LEU133 5.0 19.5 1.0 N A:ALA153 5.0 8.3 1.0

M.L.Quillin, W.A.Breyer, I.J.Griswold, B.W.Matthews. Size Versus Polarizability in Protein-Ligand Interactions: Binding of Noble Gases Within Engineered Cavities in Phage T4 Lysozyme. J.Mol.Biol. V. 302 955 2000.
ISSN: ISSN 0022-2836
PubMed: 10993735
DOI: 10.1006/JMBI.2000.4063