Krypton in PDB 1c67: T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton

All present enzymatic activity of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton:
3.2.1.17;

The structure of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton, PDB code: 1c67 was solved by M.L.Quillin, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	60.00 / 2.20
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.142, 61.142, 96.599, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The structure of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Krypton atom in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton (pdb code 1c67). This binding sites where shown within 5.0 Angstroms radius around Krypton atom.
In total 3 binding sites of Krypton where determined in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton, PDB code: 1c67:
Jump to Krypton binding site number: 1; 2; 3;

Krypton binding site 1 out of 3 in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton


Mono view


Stereo pair view

A full contact list of Krypton with other atoms in the Kr binding site number 1 of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton within 5.0Å range: probe atom residue distance (Å) B Occ A:Kr500 b:60.0 occ:0.48 KR A:KR501 2.9 60.0 0.4 KR A:KR502 2.9 60.0 0.3 CD2 A:PHE153 3.6 57.9 1.0 CB A:ALA133 3.7 26.2 1.0 CG A:PHE153 3.8 33.3 1.0 CE2 A:PHE153 4.1 58.7 1.0 CB A:PHE153 4.2 28.4 1.0 CG1 A:ILE150 4.3 23.1 1.0 CD1 A:PHE153 4.4 36.8 1.0 O A:ALA129 4.4 39.8 1.0 CB A:SER117 4.6 21.5 1.0 CZ A:PHE153 4.7 47.5 1.0 CG2 A:ILE150 4.8 32.0 1.0 SD A:MET102 4.8 30.9 1.0 CE1 A:PHE153 4.8 42.6 1.0 C A:ALA129 4.8 38.5 1.0 CA A:ALA130 4.8 27.6 1.0 CA A:ILE150 4.9 29.2 1.0 CB A:ILE150 4.9 26.4 1.0

Krypton binding site 2 out of 3 in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton


Mono view


Stereo pair view

A full contact list of Krypton with other atoms in the Kr binding site number 2 of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton within 5.0Å range: probe atom residue distance (Å) B Occ A:Kr501 b:60.0 occ:0.42 KR A:KR500 2.9 60.0 0.5 CD2 A:LEU118 3.2 39.5 1.0 CB A:SER117 3.3 21.5 1.0 N A:LEU118 3.6 43.0 1.0 C A:SER117 3.8 41.5 1.0 CA A:LEU118 3.9 52.2 1.0 CG A:LEU118 4.0 47.9 1.0 OG A:SER117 4.0 45.0 1.0 CB A:ALA121 4.2 42.3 1.0 O A:SER117 4.2 49.2 1.0 CA A:SER117 4.2 30.9 1.0 CZ A:PHE153 4.3 47.5 1.0 CE2 A:PHE153 4.4 58.7 1.0 CB A:LEU118 4.5 56.5 1.0 CB A:PHE114 4.6 29.0 1.0 CE1 A:PHE153 4.7 42.6 1.0 KR A:KR502 4.7 60.0 0.3 CD2 A:PHE153 4.8 57.9 1.0 O A:PHE114 4.8 28.6 1.0 SD A:MET102 4.9 30.9 1.0 CB A:ALA133 4.9 26.2 1.0

Krypton binding site 3 out of 3 in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton


Mono view


Stereo pair view

A full contact list of Krypton with other atoms in the Kr binding site number 3 of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Krypton within 5.0Å range: probe atom residue distance (Å) B Occ A:Kr502 b:60.0 occ:0.28 KR A:KR500 2.9 60.0 0.5 CB A:PHE153 3.4 28.4 1.0 CG A:PHE153 3.6 33.3 1.0 CD1 A:PHE153 3.8 36.8 1.0 N A:ALA130 3.9 28.2 1.0 CA A:ALA130 3.9 27.6 1.0 N A:ARG154 3.9 33.4 1.0 C A:PHE153 4.1 37.3 1.0 CB A:ALA130 4.1 22.9 1.0 O A:TRP126 4.1 59.8 1.0 CA A:ARG154 4.2 30.9 1.0 C A:ALA129 4.2 38.5 1.0 O A:ILE150 4.3 27.8 1.0 CB A:ALA129 4.3 30.1 1.0 O A:PHE153 4.4 29.2 1.0 CA A:PHE153 4.4 25.2 1.0 CB A:ARG154 4.5 35.6 1.0 CD2 A:PHE153 4.5 57.9 1.0 CG2 A:ILE150 4.6 32.0 1.0 O A:ALA129 4.6 39.8 1.0 KR A:KR501 4.7 60.0 0.4 CE1 A:PHE153 4.8 42.6 1.0 CA A:ALA129 4.9 30.2 1.0

M.L.Quillin, W.A.Breyer, I.J.Griswold, B.W.Matthews. Size Versus Polarizability in Protein-Ligand Interactions: Binding of Noble Gases Within Engineered Cavities in Phage T4 Lysozyme. J.Mol.Biol. V. 302 955 2000.
ISSN: ISSN 0022-2836
PubMed: 10993735
DOI: 10.1006/JMBI.2000.4063